Leucine Quantitative Urine 24H**

Spot Urine:10 ml of Spot urine required with clinical history and clinician number. Baby DOB, sample collection date and relevant clincial history mentioned on DBS card to be filled mandatory. Positive screen cases may require repeat testing or further workup in terms of confirmation by plasma or LCMS or GCMS method. Clinical history, metabolic disorders or inborn errors of metabolsim suspicion or provisional diagnosis of clincian required fasting recommended not mandatory. Amino acid supplementation or medications needs to stoped based on physician recommendations